The European Commission has charged Amazon with abusing its dominant position in online retail to gain an unfair advantage over competitors.
It said Amazon had used data on third-party sellers that use its marketplace to boost sales of its own-label goods.
The Commission also launched a fresh probe into the possible preferential treatment of sellers that use the tech giant's logistics services.
Amazon rejected the charges, saying no firm "cared more" for small businesses.
It faces a potential fine as high as 10% of its global turnover if it is found guilty of breaching competition law - about £15bn ($19bn).
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